The ideal combination of salts, detergents, and pH stabilisers is Tris Buffered Saline + Tween 20 (20x Concentrate), which has a pH of 7.4 and is intended to efficiently remove superfluous material from tissue samples or microtiter plate wells without interfering with the antibody binding process. Unbound components can be removed without inhibiting antigen-antibody binding interactions by preserving the ideal buffering environment. This lowers nonspecific background and raises specific signal. Hazardous preservatives like mercury or azide, which can disrupt antibody-antigen binding interactions, are absent from our wash buffers. Wash buffer is available in numerous formulations to suit the requirements of your particular ELISA application for your convenience.