Actin Smooth Muscle (SMA) Antibody (1A4)

Actin, Smooth Muscle (SMA) Antibody

Clone 1A4

The Actin Smooth Muscle (SMA) Antibody, clone 1A4, is a widely trusted mouse monoclonal antibody designed for the specific detection of α-smooth muscle actin (α-SMA), a 42 kDa cytoskeletal protein prominently expressed in smooth muscle cells, myofibroblasts, pericytes, and myoepithelial cells. Clone 1A4 is one of the most commonly used antibodies in both diagnostic and research settings for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissues. This antibody delivers strong cytoplasmic staining with high specificity and minimal background, making it an essential marker for identifying smooth muscle tumors, such as leiomyomas, leiomyosarcomas, and gastrointestinal stromal tumors (GISTs). Additionally, it plays a key role in evaluating stromal myofibroblastic reactions in carcinomas, aiding in tumor characterization and microenvironment assessment. SMA clone 1A4 is also critical in identifying vascular structures, due to its ability to highlight vascular smooth muscle cells and pericytes, thus aiding in studies of angiogenesis, fibrosis, wound healing, and chronic inflammation. Its robust performance and compatibility with automated staining platforms make it a reliable tool for both routine pathology and translational research. With proven utility across a wide range of tissue types, the SMA antibody (1A4) is an essential reagent in oncology, cardiovascular research, pulmonary pathology, and soft tissue tumor diagnostics.