The BCL-6 (B-cell lymphoma 6) Rabbit Monoclonal Antibody from GeneBioSolution is a premium-grade immunohistochemistry (IHC) antibody developed for the accurate and reproducible detection of BCL-6 protein in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Manufactured under ISO 13485-certified conditions and optimized for both manual and automated IHC platforms, this antibody offers exceptional specificity, strong nuclear staining, and minimal background, making it an indispensable marker in hematopathology diagnostics. BCL-6 is a nuclear transcription repressor belonging to the zinc finger protein family, crucial for germinal center B-cell development and regulation. It plays a vital role in the suppression of genes involved in B-cell activation and terminal differentiation. Abnormal expression of BCL-6 is strongly associated with germinal center-derived B-cell lymphomas, including diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. Detection of BCL-6 via IHC is an essential tool for subclassifying lymphomas and guiding patient prognosis. The GeneBioSolution BCL-6 Rabbit Monoclonal Antibody delivers a clear nuclear staining pattern in positive cells, providing pathologists with high-confidence interpretation. Its high affinity and optimized formulation ensure consistent performance across a range of tissue types. The antibody is particularly valuable for identifying germinal center B-cells in both normal and neoplastic lymphoid tissues, and for aiding in the molecular subclassification of DLBCL into germinal center B-cell-like (GCB) and activated B-cell-like (ABC) types. Product Features & Advantages: Antibody Type: Rabbit monoclonal – high affinity and specificity Target: BCL-6 protein, nuclear transcription repressor Cellular Localization: Nuclear staining Applications: IHC on FFPE human tissue Formats Available: Ready-to-use (RTU) for streamlined workflows and Concentrated for custom protocol optimization Compatibility: Works with leading automated IHC stainers and manual systems Quality Control: Validated on lymphoma, tonsil, and reactive lymphoid tissue. Recommended Use & Protocol Highlights: For optimal staining, use heat-induced epitope retrieval (HIER) with EDTA buffer (pH 8.0–9.0), followed by detection with a polymer-based high-sensitivity detection system. Recommended positive control tissues include tonsil or known BCL-6-positive lymphoma samples. Key Applications in Pathology: Diagnosis and subclassification of B-cell lymphomas Identification of germinal center B-cell populations in normal and malignant tissues Prognostic stratification of DLBCL cases Research into transcriptional regulation and immune response pathways Quality Assurance: Each lot of GeneBioSolution BCL-6 Rabbit Monoclonal Antibody undergoes multi-tier validation that includes tissue microarray testing, reproducibility analysis, and independent inter-laboratory performance evaluation. Manufactured in compliance with international quality standards
Humans; others not tested
Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Buffer with protein carrier and preservative
Store at 2ºC to 8ºC
Tonsil or diffuse large B-cell lymphoma
Nuclear
IgG
Human BCL-6 protein fragment (aa256-389)
At Genebiosolution, our commitment lies in crafting cutting-edge solutions for researchers, clinicians, and diagnostic laboratories seeking excellence in immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). With a focus on quality, reliability, and accuracy, our products empower advancements in molecular diagnostics and research methodologies.
