BCL 6 Antibody (mouse monoclonal)

BCL-6 Antibody (mouse monoclonal)

The BCL-6 (B-cell lymphoma 6) Mouse Monoclonal Antibody from GeneBioSolution is a high-performance immunohistochemistry (IHC) reagent designed for precise and reproducible detection of BCL-6 protein in formalin-fixed, paraffin-embedded (FFPE) tissue sections. Manufactured under ISO 13485-certified processes and optimized for both manual and automated IHC staining platforms, this antibody delivers excellent specificity, strong signal intensity, and minimal background, making it an essential biomarker for hematopathology diagnostics. BCL-6 is a nuclear zinc finger transcription factor that plays a critical role in the regulation of germinal center B-cell development and differentiation. It is essential for the formation and maintenance of germinal centers during the immune response and functions by repressing genes involved in B-cell activation and terminal differentiation. Abnormal expression of BCL-6 is closely associated with germinal center-derived B-cell lymphomas, including diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma, and is a valuable marker in the differential diagnosis of B-cell malignancies. The GeneBioSolution BCL-6 Mouse Monoclonal Antibody exhibits a distinct nuclear staining pattern in positive cells, providing clear and interpretable results in both neoplastic and reactive tissues. It is highly effective for identifying germinal center B-cells in lymphoid tissues and for subclassifying DLBCL into germinal center B-cell-like (GCB) and activated B-cell-like (ABC) subtypes, which have important prognostic implications. Product Features & Advantages: Clone: High-affinity mouse monoclonal antibody Target: BCL-6 transcription repressor protein Cellular Localization: Nuclear staining Applications: IHC on FFPE tissues Formats Available: Ready-to-use (RTU) for workflow efficiency and Concentrated for customized protocol development Compatibility: Suitable for major automated IHC platforms and manual staining methods Quality Control: Validated on a wide range of lymphoma and reactive lymphoid tissues; Recommended Use & Protocol Highlights: For optimal results, antigen retrieval is recommended using heat-induced epitope retrieval (HIER) in EDTA buffer (pH 8.0–9.0). Detection with a high-sensitivity polymer detection system is advised to achieve crisp, high-contrast nuclear staining. Positive control tissues include tonsil and known BCL-6-positive lymphoma samples. Key Applications in Pathology: Differential diagnosis of B-cell lymphomas Identification of germinal center B-cells in reactive and neoplastic lymphoid tissues Subclassification of diffuse large B-cell lymphoma (DLBCL) into prognostically significant subtypes Research on transcriptional regulation and germinal center biology Quality Assurance: Every batch of GeneBioSolution BCL-6 Mouse Monoclonal Antibody is subjected to rigorous validation, including testing on tissue microarrays, reproducibility checks across multiple staining runs, and inter-laboratory performance evaluation. With its proven diagnostic utility, reproducible performance, and compatibility with modern IHC workflows, the GeneBioSolution BCL-6 Mouse Monoclonal Antibody is an indispensable tool for pathologists and researchers seeking accurate identification of germinal center B-cell lineage and related malignancies.