Biotin Mouse IgG Rabbit Monoclonal Antibody – GeneBioSolution The Biotin Mouse IgG Rabbit Monoclonal Antibody from GeneBioSolution is a high-performance, biotin-conjugated secondary antibody designed for sensitive, specific, and reproducible detection of mouse immunoglobulin G (IgG) in a wide range of immunoassay applications. Manufactured under ISO 13485-certified quality systems, this antibody delivers strong binding affinity, low background, and consistent results for both research and diagnostic use. Product Overview Mouse IgG antibodies are widely used as primary antibodies in immunohistochemistry (IHC), ELISA, immunofluorescence (IF), and other immunoassay platforms. This rabbit monoclonal anti-mouse IgG is conjugated to biotin, enabling high-affinity interaction with streptavidin or avidin-based detection systems. The result is enhanced signal amplification, ideal for detecting low-abundance targets or achieving maximum contrast in staining applications. GeneBioSolution’s proprietary conjugation process is optimized to preserve the antibody’s specificity and functional integrity, ensuring that the binding to mouse IgG remains strong and selective, with minimal cross-reactivity to other immunoglobulin classes. Applications Immunohistochemistry (IHC) – Detection of mouse IgG primary antibodies in FFPE tissue sections ELISA – Signal amplification for mouse IgG detection in microplate assays Immunofluorescence (IF) – High-sensitivity imaging using fluorescent streptavidin conjugates Western Blot (WB) – Enhanced detection of mouse IgG-bound proteins via biotin-streptavidin systems Flow Cytometry (FC) – Amplified detection in flow-based assays Features & Benefits High Specificity – Minimal cross-reactivity with non-mouse immunoglobulins Biotin Conjugation – Strong, stable binding to streptavidin/avidin detection reagents Rabbit Monoclonal Production – High affinity, reproducibility, and lot-to-lot consistency Versatile Formats – Available in Ready-to-Use (RTU) and Concentrated solutions Recommended Use Working Dilution: IHC (1:50–1:200); ELISA (1–5 µg/mL) depending on assay conditions Antigen Retrieval: As per the protocol for the mouse IgG primary antibody target Detection System: Compatible with streptavidin-HRP, streptavidin-AP, and streptavidin-fluorophore systems Positive Controls: Known tissue or assay containing mouse IgG primary antibody staining Quality Control & Validation Each lot undergoes rigorous QC testing, including: Purity assessment by SDS-PAGE Determination of biotin-to-antibody ratio Functional performance in IHC and ELISA Lot-to-lot reproducibility analysis Ordering Information Product Name: Biotin Mouse IgG Rabbit Monoclonal Antibody Conjugate: Biotin Host: Rabbit monoclonal Target: Mouse IgG Applications: IHC, ELISA, IF, WB, Flow Cytometry Formats: RTU and Concentrated With its superior binding performance, reliable signal amplification, and compatibility with multiple assay platforms, the GeneBioSolution Biotin Mouse IgG Rabbit Monoclonal Antibody is the ideal choice for laboratories seeking precision, reproducibility, and high sensitivity in mouse IgG detection.
RM104
RM104 reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG. The Fc region of RM104 has been engineered to eliminate Fc receptor binding.
Mouse IgG
ELISA Western Blot (WB) (nonreduced)
Mouse
Biotin
Research Use Only
Store at -20°C. 6 Months, Avoid Freeze and Thaw Cycles
At Genebiosolution, our commitment lies in crafting cutting-edge solutions for researchers, clinicians, and diagnostic laboratories seeking excellence in immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). With a focus on quality, reliability, and accuracy, our products empower advancements in molecular diagnostics and research methodologies.
