CD20 Antibody (Mouse Monoclonal)

CD20 Antibody (Mouse Monoclonal)

CD20 Antibody [L26] – Mouse Monoclonal Antibody for Human B Cell Detection The CD20 antibody [L26] is a highly specific mouse monoclonal antibody used for the detection of human CD20, a cell surface phosphoprotein expressed on B lymphocytes. This antibody is widely used in immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissues, as well as in flow cytometry and other immunoassays for B-cell lineage identification and diagnostic pathology. Clone L26 is one of the most established and validated clones for CD20 detection, known for its high specificity, strong membrane staining, and minimal background. It is particularly useful in the diagnosis of B-cell lymphomas, such as diffuse large B-cell lymphoma (DLBCL), follicular lymphoma, and chronic lymphocytic leukemia (CLL), where CD20 expression serves as a critical biomarker. Key Features and Benefits: Clone: L26 Host Species: Mouse Isotype: IgG2a Reactivity: Human CD20 antigen Applications: Immunohistochemistry (IHC, FFPE) Subcellular Localization: Cell membrane Recommended Controls: Human tonsil or lymph node tissue Available Formats: Unconjugated, HRP-conjugated, FITC, PE, and others CD20 Antigen Background: CD20 is a 33–37 kDa non-glycosylated phosphoprotein encoded by the MS4A1 gene. It is expressed on pre-B and mature B cells, but not on early pro-B cells or plasma cells. CD20 functions as a calcium channel involved in B-cell activation and proliferation. Because CD20 is not internalized upon antibody binding, it is a key therapeutic and diagnostic target in B-cell malignancies. The L26 clone is commonly used to determine CD20 positivity in hematopathology, guiding both diagnosis and potential therapeutic approaches such as anti-CD20 monoclonal antibody therapy (e.g., rituximab). Why Choose Clone L26 Extensive clinical and research validation High sensitivity and specificity for B cells Trusted in diagnostic and academic laboratories worldwide Compatible with automated IHC platforms Applications in Research and Diagnostics: Classification of B-cell lymphomas Evaluation of lymphoid infiltrates Study of B-cell immunophenotyping in autoimmune diseases Biomarker analysis in clinical pathology