CD20 Antibody (Rabbit Monoclonal)

CD20 Antibody (Rabbit Monoclonal)

The CD20 Antibody (Rabbit Monoclonal), clone RM272, is a highly specific and sensitive antibody designed for the detection of the human CD20 antigen, a transmembrane phosphoprotein prominently expressed on the surface of B lymphocytes throughout most stages of B-cell development. This antibody clone is engineered in rabbit to provide enhanced affinity and specificity compared to traditional mouse monoclonal antibodies, resulting in improved staining quality and reproducibility. Clone RM272 is extensively validated for immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissues, flow cytometry (FC), and other immunodetection techniques. It is widely used in both research and clinical pathology for the identification, classification, and study of B-cell populations and B-cell malignancies including diffuse large B-cell lymphoma (DLBCL), follicular lymphoma, and chronic lymphocytic leukemia (CLL). CD20 is a non-glycosylated phosphoprotein expressed on the surface of pre-B cells, immature and mature B cells but absent on plasma cells and stem cells. It functions as a calcium channel involved in B-cell activation, proliferation, and differentiation. CD20 is an important biomarker in immunophenotyping of B-cell lineage and is frequently targeted in therapeutic antibody treatments such as rituximab and ofatumumab. The RM272 clone, generated in rabbit, delivers superior binding affinity and high specificity for human CD20, enabling crisp membrane staining with minimal background noise. This clone's performance in IHC and flow cytometry assays supports reliable detection of both normal and malignant B cells, critical for research in immunology, oncology, and hematopathology.