The CD25 Antibody (Mouse Monoclonal, Clone 4C9) is a highly specific reagent targeting the interleukin-2 receptor alpha chain (IL-2Rα or CD25), which is upregulated on activated T cells, regulatory T cells (Tregs), and certain hematologic malignancies. CD25 plays a pivotal role in T-cell activation, immune regulation, and autoimmune response, making it a critical biomarker in both immunological research and oncology diagnostics. Clone 4C9 is a well-validated mouse monoclonal antibody that delivers reliable and reproducible staining in flow cytometry, immunohistochemistry (IHC) on formalin-fixed paraffin-embedded (FFPE) tissues, and immunofluorescence (IF). It is especially useful for identifying CD25-positive lymphoid populations in lymph nodes, thymus, and tumor samples, supporting the diagnosis and monitoring of conditions such as Hodgkin lymphoma, adult T-cell leukemia/lymphoma (ATLL), and autoimmune diseases. With high affinity, minimal background staining, and consistent performance across platforms, the CD25 Mouse Monoclonal Antibody Clone 4C9 is an essential tool for researchers and clinicians studying T-cell dynamics, immune checkpoint regulation, and therapeutic antibody development.
Mouse
Recombinant fragment of human IL2RA protein (around aa 42-183) (exact sequence is proprietary)
IL2RA/2395
IgG2b/Kappa
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
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