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Citrate Buffer (10x) HIER Solution, pH 6.0

Citrate Buffer (10x) HIER Solution, pH 6.0, is a concentrated antigen retrieval buffer widely used in histology and immunohistochemistry (IHC) for Heat-Induced Epitope Retrieval (HIER). Designed to be diluted to working strength before use, this 10x citrate buffer maintains a stable, mildly acidic pH of 6.0, which is optimal for effectively unmasking antigenic sites in formalin-fixed, paraffin-embedded (FFPE) tissue sections. By breaking protein cross-links formed during formalin fixation, the citrate buffer enhances antibody binding, significantly improving staining intensity, specificity, and reproducibility in immunostaining protocols. This citrate-based HIER solution is highly favored by researchers and diagnostic labs due to its proven compatibility with a broad range of primary antibodies, detection systems, and tissue types. Its reliable performance plays a crucial role in achieving high-quality immunohistochemical results for both clinical diagnostics and biomedical research. Available as a concentrated 10x solution, it offers convenience and flexibility, allowing precise dilution for various laboratory protocols requiring antigen retrieval.

Available Sizes

GCB500: 500 mL
GCB999: 1000 mL

Ordering Information

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  • Classification
  • RUO
  • Catalogue Number
  • GCBRP