Human IgG (Immunoglobulin G) is the predominant antibody isotype in human serum, responsible for the majority of antibody-based immune responses. The biotin conjugation of Human IgG allows it to interact with avidin/streptavidin conjugates for enhanced detection sensitivity in multiple assay formats, including ELISA, IHC, immunofluorescence, and Western blotting. GeneBioSolution’s Human IgG Biotin is manufactured under ISO 13485-certified conditions, ensuring lot-to-lot consistency, high purity, and reproducible results. The biotinylation process is carefully optimized to preserve the structural integrity and antigen-binding activity of the antibody. Applications Immunohistochemistry (IHC) – for detection of antigens in FFPE tissues using biotin-streptavidin amplification ELISA & Immunoassays – as a positive control or in detection systems requiring biotin-labeled antibodies Immunofluorescence (IF) – for enhanced signal in cell and tissue staining Western Blot – as a biotin-labeled control antibody Flow Cytometry – for signal amplification in low-abundance targets Features & Benefits High-Purity IgG – purified from human serum and conjugated with biotin using optimized chemistry Consistent Performance – validated across multiple immunoassay formats Strong Signal Amplification – ideal for applications requiring high sensitivity Multiple Formats – available in ready-to-use (RTU) and concentrated solutions
RM116
RM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Human IgG
Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA
Human
Biotin
Research Use Only
Stable for 1 Year at -20.0°C from date of receipt.