The Involucrin Monoclonal Antibody [Clone SY5] is a mouse IgG1 antibody that specifically detects human involucrin, a protein essential for the terminal differentiation of squamous epithelial cells. This antibody shows strong cytoplasmic reactivity in the upper layers of stratified squamous epithelium and is validated for use in immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded tissues. Clone SY5 is a reliable tool for distinguishing squamous cell carcinomas, evaluating keratinocyte maturation, and studying epidermal biology.
Mouse
Purified involucrin from human keratinocytes
SY5
IgG1, kappa
Human, Gorilla, Owl monkey, Pig, and Dog. Does not react with Mouse. Others not known.
MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
This antibody recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 120kDa protein.
Cytoplasmic