Retinoblastoma (RB)

Product Overview

Retinoblastoma (RB) antibody clone GR444 is a mouse monoclonal antibody developed to detect the retinoblastoma protein (pRB), a crucial tumor suppressor involved in the regulation of the cell cycle. The RB protein plays a pivotal role in controlling cell proliferation by inhibiting the transition from the G1 to the S phase of the cell cycle through its interaction with E2F transcription factors. Loss or inactivation of pRB is commonly associated with a variety of human cancers, including the pediatric ocular malignancy retinoblastoma, as well as osteosarcomas and certain epithelial tumors. Clone GR444 specifically recognizes the underphosphorylated, active form of pRB, making it a valuable tool in distinguishing between functionally active and inactive states of the protein in tissue samples. This antibody is widely used in immunohistochemistry (IHC) to assess RB protein expression and status in tumor diagnostics, particularly in evaluating RB pathway disruptions. Its high specificity and strong nuclear staining pattern allow for precise localization and interpretation in both research and clinical pathology settings.