The RNA Polymerase II Antibody is a high-affinity reagent developed to detect RNA Polymerase II (Pol II), the essential DNA-dependent RNA polymerase responsible for synthesizing messenger RNA (mRNA) and numerous non-coding RNAs in eukaryotic cells. Pol II plays a central role in transcription initiation, elongation, and RNA processing, with activity tightly regulated by the phosphorylation state of its C-terminal domain (CTD) heptapeptide repeats (YSPTSPS). Dysregulation of Pol II has been implicated in transcriptional reprogramming associated with oncogenesis, neurodegeneration, and developmental disorders. This antibody provides strong nuclear staining in immunohistochemistry (IHC) and highly specific detection in western blotting (WB), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF). It enables precise evaluation of Pol II expression and phosphorylation status, supporting research into gene regulation, epigenetic control, transcriptional pausing, and elongation dynamics. In pathology, RNA Polymerase II immunodetection is useful for characterizing tumor transcriptional activity, with aberrant Pol II signaling linked to multiple cancers, including glioblastoma, breast carcinoma, and hematological malignancies.
Mouse
Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
CTD4H8
IgG1 / Kappa
Human, Mouse, Rat
HAP1, HePG2, K562, NIH3T3 cells. Human testis., PC3
Recognizes RNA polymerase II (pol II), an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-encoded genes.
Chromosome, Cytoplasm, Nucleus
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