GeneBio’s rabbit recombinant antibody recognizes SDHB (Succinate dehydrogenase B), an iron-sulfur subunit of mitochondrial complex II, a vital component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane.
Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. Additionally SDHB mutations are associated with a subset of RCC and GIST tumors including Carney-Stratakis Syndrome or Carney Triad that exhibit a loss of SDHB expression.
Loss of SDHB may indicate asyndromic disease caused by either germline mutation or SDHC hypermethylation which is found in a subset of gastrointestinal stromal tumors. Immunohistochemistry for SDHB is a reliable and inexpensive surrogate for assessment of syndromic loss of succinate dehydrogenase. Additionally, the loss of SDHB is seen in about 15% of pheochromocytomas and paragangliomas, 3% of gastrointestinal stromal tumors, 1% of renal cell carcinomas and 1% of pituitary adenomas.
Humans; others not tested
Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Buffer with protein carrier and preservative
Store at 2ºC to 8ºC
Paraganglioma
Cytoplasmic
IgG
Synthetic peptide corresponding to residues in human SDHB was used as an immunogen
