Description

The Genebio ERBB3/CEN 12 Dual Color Probe is designed for the detection of amplifications of the chromosomal regions harboring the ERBB3 gene. It belongs to the epidermal growth factor receptor subgroup of the receptor tyrosine kinase superfamily which is known to be affected by gene amplifications in several malignant tumors.

What is the purpose of the Genebio ERBB3/CEN 12 dual color probe?

The Genebio ERBB3/CEN 12 dual color probe serves a specific purpose in the field of molecular biology and genetics. This probe is designed to facilitate the detection and analysis of specific genetic abnormalities associated with the ERBB3 gene and the CEN 12 region.

The ERBB3 gene, also known as HER3, is an important member of the ErbB family of receptor tyrosine kinases. It plays a crucial role in signaling pathways and is implicated in various biological processes, including cell growth, differentiation, and survival. Abnormalities in the ERBB3 gene have been linked to the development and progression of certain types of cancer, such as breast, ovarian, and lung cancer.

On the other hand, CEN 12 refers to the centromere region of chromosome 12. The centromere is a specialized region of the chromosome that plays a critical role in cell division and the accurate segregation of chromosomes during mitosis and meiosis. Aberrations in the CEN 12 region can lead to chromosomal instability and genomic imbalances, which can have significant implications in the development of genetic disorders and cancer.

The Genebio ERBB3/CEN 12 dual color probe is specifically designed to target and bind to the ERBB3 gene and the CEN 12 region simultaneously. It utilizes two different fluorescent dyes, each emitting a distinct color when illuminated by specific wavelengths of light. By employing this dual color probe, researchers can visually and quantitatively assess the presence and localization of both the ERBB3 gene and the CEN 12 region in a sample.

One of the key applications of the Genebio ERBB3/CEN 12 dual color probe is in fluorescence in situ hybridization (FISH) assays. FISH is a powerful molecular technique that allows researchers to visualize and map the location of specific DNA sequences within the cells. It involves the use of fluorescently labeled probes that can bind to complementary target DNA sequences.

In the case of the ERBB3/CEN 12 dual color probe, it enables researchers to simultaneously detect and analyze the ERBB3 gene and the CEN 12 region. The probe is designed to hybridize or bind specifically to the target DNA sequences, and the fluorescent dyes attached to the probe emit distinct colors when excited by specific wavelengths of light. This dual-color labeling allows for easy differentiation and localization of the ERBB3 gene and the CEN 12 region within the cell nucleus.

Furthermore, the Genebio ERBB3/CEN 12 dual color probe provides valuable information about the genetic abnormalities associated with the ERBB3 gene and the CEN 12 region. By examining the presence, quantity, and distribution of these specific DNA sequences, researchers can gain insights into the genetic changes occurring in cells. This information can be used to study the mechanisms underlying diseases such as cancer and genetic disorders.

How does the Genebio ERBB3/CEN 12 dual color probe work?

The Genebio ERBB3/CEN 12 dual color probe is a powerful tool used in genetic research to detect specific genes and chromosomal regions. This advanced probe utilizes two distinct fluorescent dyes to visualize the ERBB3 gene and the CEN 12 chromosomal region simultaneously.

To understand how this probe works, let’s break it down into two parts: the ERBB3 gene detection and the CEN 12 chromosomal region detection.

1. ERBB3 gene detection: The ERBB3 gene, also known as HER3, is an important gene involved in cell growth and development. It is often implicated in various diseases, including cancer. The ERBB3/CEN 12 dual color probe specifically targets the ERBB3 gene using a complementary DNA sequence that is labeled with a fluorescent dye, such as green.When the probe is introduced to a sample containing DNA, it will bind to the specific complementary sequence present in the ERBB3 gene. The green fluorescence emitted by the dye allows researchers to visualize the presence and location of the ERBB3 gene within the sample. This detection method is highly specific, ensuring that only the ERBB3 gene is visualized.
2. The Genebio ERBB3/CEN 12 dual color probe is a powerful tool in the field of biological research and analysis. It is specifically designed for the detection of genetic abnormalities and alterations in the ERBB3 gene and the centromere region of chromosome 12. This probe is primarily used in fluorescence in situ hybridization (FISH) assays, which allow for the precise visualization and analysis of specific DNA sequences within cells.
3. The ERBB3 gene, also known as HER3, plays a crucial role in cell signaling pathways and is associated with various diseases, including cancer. The CEN 12 region, on the other hand, represents the centromere of chromosome 12, which is essential for proper chromosome segregation during cell division.
4. With the Genebio ERBB3/CEN 12 dual color probe, researchers can analyze a wide range of biological samples to investigate genetic alterations. These samples can include:
5. Tissue sections: The dual color probe can be used on fixed tissue sections obtained from biopsies or surgical specimens. This allows for the examination of the ERBB3 gene and chromosome 12 abnormalities within the specific tissue of interest.
6. Cell lines: Researchers often use cultured cell lines as models for studying genetic changes. The ERBB3/CEN 12 dual color probe can be applied to these cells to assess genetic alterations associated with ERBB3 and chromosome
7. Blood samples: Peripheral blood samples can be analyzed using the dual color probe to identify genetic abnormalities in the ERBB3 gene and the CEN 12 region. This can be particularly useful in diagnosing genetic disorders and monitoring disease progression.
8. The Genebio ERBB3/CEN 12 dual color probe works by utilizing the technique of fluorescence in situ hybridization (FISH). In FISH, fluorescently labeled probes are used to bind to specific DNA sequences within cells. These probes emit distinct colors when excited by specific wavelengths of light, allowing for the visualization and mapping of the target DNA sequences.
9. In the case of the Genebio ERBB3/CEN 12 dual color probe, it uses two distinct fluorescent dyes to simultaneously detect the ERBB3 gene and the CEN 12 region. The probe is designed to bind specifically to the target DNA sequences in these regions. When the probe is introduced to a sample containing DNA, it will bind to the complementary sequences in the ERBB3 gene and the CEN 12 region. The resulting fluorescence allows researchers to easily differentiate and localize these genetic regions within the cell nucleus.
10. By examining the presence, quantity, and distribution of the ERBB3 gene and the CEN 12 region, researchers can gain valuable insights into genetic changes associated with diseases such as cancer. This information can contribute to a better understanding of disease mechanisms and potentially guide the development of targeted therapies.

Can the Genebio ERBB3/CEN 12 dual color probe be used in diagnostic applications?

Unlocking Diagnostic Potential: Exploring the Genebio ERBB3/CEN 12 Dual Color Probe

In the realm of diagnostic applications, accuracy and reliability are paramount. As nature enthusiasts and professionals in the field, we understand the importance of staying abreast of cutting-edge technologies that can aid in accurate diagnoses. In this blog post, we will delve into the potential of the Genebio ERBB3/CEN 12 dual color probe and its suitability for diagnostic applications.

Understanding the Genebio ERBB3/CEN 12 Dual Color Probe: The Genebio ERBB3/CEN 12 dual color probe is a sophisticated tool designed for genomic analysis. Specifically, it targets the ERBB3 gene, which is associated with various diseases, including cancer, and the CEN 12 region, which serves as a centromeric marker for chromosome 12.

Diagnostic Applications: While the Genebio ERBB3/CEN 12 dual color probe is primarily used in research settings, its potential for diagnostic applications cannot be overlooked. It offers several features that make it a compelling tool for accurate diagnoses, including:

1. High Sensitivity: The dual color probe exhibits exceptional sensitivity, enabling the detection of even minute genetic aberrations associated with diseases. This precision is crucial for accurate diagnosis and treatment planning.
2. Simultaneous Visualization: By targeting both the ERBB3 gene and the CEN 12 region, this probe allows for concurrent assessment of chromosomal abnormalities and gene amplification in a single sample. This simultaneous visualization provides a comprehensive view of the genetic alterations, aiding in the accurate diagnosis and understanding of disease progression.
3. Versatility: The Genebio ERBB3/CEN 12 dual color probe can be used with various biological samples, including tissue sections, cell lines, and blood samples. This versatility allows for its application in a wide range of diagnostic scenarios.4. Specificity: One of the key strengths of the Genebio ERBB3/CEN 12 dual color probe is its high specificity. It is designed to bind exclusively to the target DNA sequences in the ERBB3 gene and the CEN 12 region, ensuring accurate and reliable results. This specificity minimizes the risk of false-positive or false-negative findings, which is crucial in diagnostic applications.