Citrate Buffer (10x) HIER Solution, pH 6.0, is a concentrated buffer solution commonly used in histology and immunohistochemistry for Heat-Induced Epitope Retrieval (HIER). It is prepared at a 10-fold concentration to allow dilution to working strength before use. The buffer is carefully formulated to maintain a stable pH of 6.0, which is optimal for unmasking antigenic sites in formalin-fixed, paraffin-embedded tissue sections during the antigen retrieval process. This acidic environment helps break protein cross-links formed during fixation, enhancing antibody binding and improving staining intensity and specificity. The citrate-based solution is widely favored due to its effectiveness, reproducibility, and compatibility with a broad range of antibodies and detection systems. Its role is critical in ensuring high-quality immunostaining results for diagnostic and research applications.
GCB500: 500 mL
GCB999: 1000 mL